Recombinant Mouse Anti-HCV E2 Antibody (AP33)
CAT#: PABL-496
Recombinant Mouse Antibody (AP33) is capable of binding to HCV E2 , expressed in Chinese Hamster Ovary cells (CHO). This mouse monoclonal antibody recognizes a conserved, linear epitope on E2 and potently neutralizes a broad range of HCV genotypes.
Published Data
Tested Data
FC
Figure 1 Inhibition of HCV-LPs binding to cells by anti-E1 and anti-E2 antibodies.
SYTO-labeled HCV-LPs were pre-incubated with 20 to 100μg of anti-E2 (AP33) for 2h at 4°C. The HCV-LP-antibody mixtures were then incubated with Molt-4 cells for 1 h. Flow cytometry histogram of HCV-LPs binding inthe presence (20 μg/ml) (open graph) or absence (black graph) of antibodies. The background binding is shown as the gray graph.
Triyatni, M., Saunier, B., Maruvada, P., Davis, A. R., Ulianich, L., Heller, T., ... & Liang, T. J. (2002). Interaction of hepatitis C virus-like particles and cells: a model system for studying viral binding and entry. Journal of virology, 76(18), 9335-9344.
Neut
Figure 2 Neutralization of HCVpp of genotype 1a using AP33 and e509 combinations.
Increasing concentrations of AP33 alone and in combination with e509 mAb at a fixed saturating concentration (10 μg/mL). The concentration of each mAb, alone or in combination, is reported on x axis. The neutralizing activity (y axis) is expressed as percent reduction infectivity of HCVpp of genotype 1a (H77 strain) without mAbs.
Sautto, G., Mancini, N., Diotti, R. A., Solforosi, L., Clementi, M., & Burioni, R. (2012). Anti-hepatitis C virus E2 (HCV/E2) glycoprotein monoclonal antibodies and neutralization interference. Antiviral research, 96(1), 82-89.
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SDS-PAGE
Figure 1 Anti-Human CEACAM5 Recombinant Antibody (PABL-496) in SDS-PAGE
SDS-PAGE analysis of PABL-496 in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions.
SEC-HPLC
Figure 2 Anti-Human CEACAM5 Recombinant Antibody (PABL-496) in SEC-HPLC
The purity of PABL-496 was 96.2631% as determined by SEC-HPLC.
WB
Figure 3 Anti-Human CEACAM5 Recombinant Antibody (PABL-496) in WB
Western blot analysis of PABL-496 was performed by loading HCV E2 Protein (His Tag) protein, His tag.
ELISA
Figure 4 Anti-Human CEACAM5 Recombinant Antibody (PABL-496) in ELISA
ELISA analysis of PABL-496 was performed by coating with HCV E2 Protein (His Tag). Then blocked with BSA and incubated with PABL-496. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Immunogen
- HCV E2 envelope glycoprotein
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native HCV E2
- Species Reactivity
- HCV
- Clone
- AP33
- Applications
- FC, Neut, FuncS
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This antibody has been reported for use in Neutralization, Flow Cytometry and Function Assay.
Target
- Alternative Names
- E2; E2 (HCV); HCV E2
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(Creative Biolabs Cat# PABL-496, RRID: AB_3111660)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
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